To detect pathogens of venereal, dermatological, gynecological, urological and other diseases, a method called bacteriological culture is used.
Technology of analysis
Biomaterial is placed in a favorable environment created in the laboratory. After a few days or weeks, it "grows" with microorganisms, which are subsequently tested for sensitivity to antibiotics and antimicrobial agents. The result of bacterosseum is an antibioticogram showing which preparation the agent is most afraid of. Based on this information, treatment is prescribed.
Why Bucks?
The analysis is widely used to identify pathogens of various infections, including venereal diseases, diseases of the genitourinary system, hearing and respiratory organs, various kinds of inflammation.
Back-up on the microflora helps identify the pathogen and determine the most effective means of combating it. Disadvantages of the method:
- duration of implementation;
- the need for complete sterility when taking the biomaterial under study;
- the results of bakposeve may be inaccurate due to the long transportation of the material or insufficient qualification of the laboratory assistant.
The measurement of the concentration of microorganisms in the material is measured in CFU / ml (colony forming units).
Urine boiling
The analysis is conducted to identify the causative agent of urinary infections. The biomaterial is fresh urine collected in a sterile container (stored for no more than 2 hours at 15-25 ° C).
Before taking the urine, you must thoroughly wash the external genitalia.
The presence of microorganisms in the urine in an amount of less than 103 cfu / ml indicates a healthy microflora. The result above 105 cfu / ml indicates the presence of the pathogen that caused the inflammatory process.
Bakposose from the cervical canal
Biomaterial is taken from the cervix, analysis is shown:
- pregnant women;
- Patients in whom smear on the flora were found gram-negative diplococci;
- inflammatory processes in the pelvic organs;
- with vulvovaginitis of a chronic or recurrent form.
Also, the material for plant seeding on the microflora is taken from the vagina and urethra. The analysis helps to identify trichomoniasis, tuberculosis, gonorrhea, mycoplasmosis and other diseases caused by pathogenic microorganisms. In the same way diagnosed ureplazmoz - bakposev on ureaplasma is done on the basis of samples from the vaults of the vagina, cervix and urethral mucosa.
Nasal and tonsil alimentary cuff
The analysis is carried out with suspicion of the bacterial nature of sinusitis, rhinitis and pharyngitis and helps to identify pneumococcal, staphylococcal and streptococcal infections. To identify hemolytic group A streptococci, bacterial bacaps from the throat are made.
The fence is carried out 2 hours after a meal or on an empty stomach with sterile swabs from the surface of the tonsils and the nasal mucosa.
Bloodsucking Blood
The analysis is shown seriously ill with chills and fever, as well as patients with suspected immunosuppression, endocarditis or intravascular infection. For bacterosseous, the blood is taken from both hands at an interval of 30 minutes, the test tube is introduced into a bottle with a nutrient medium.
The material should be taken at the peak of temperature (heat) before taking antimicrobials.
Normally, the blood should be sterile.
Back-up from the ear
The analysis allows to identify the causative agents of the inflammatory processes of the inner, middle or outer ear. Preparation for bacteriosum is discussed with the doctor - it is necessary to do the analysis before the beginning of antimicrobial therapy.
The norm is the presence in the biomaterial of coagulase-negative staphylococci and diphtheria (skin dwellers).